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zymobiomics microbial community standard  (Zymo Research)


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    Zymo Research zymobiomics microbial community standard
    Zymobiomics Microbial Community Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zymobiomics+microbial+community+standard/ZymoBIOMICS+Microbial+Community+Standard/pm42905466-78-10-14
    Average 99 stars, based on 919 article reviews
    zymobiomics microbial community standard - by Bioz Stars, 2026-11
    99/100 stars

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    DNA Extraction:

    Article Title: Oral microbiome and postmenopausal breast cancer risk: findings from the NIH-AARP diet and health study
    Article Snippet: .. A total of 15 DNA extraction batches were created with matched case-control pairs included in the same batch along with 6 quality control samples: two bacterial artificial communities (the Microbiome Quality Control Project [MBQC] oral artificial community [ ] and the ZymoBiomics Microbial Community Standard [Zymo Research, Irvine, CA]), a mouthwash from a generous donor, two extraction replicates and one extraction blank (TE buffer). .. DNA extractions were performed at the Cancer Genomics Research Laboratory (CGR) at the National Cancer Institute (NCI) in Rockville, Maryland, using the DNeasy PowerSoil Pro kit on the QIAcube HT automated purification system (Qiagen, Hilden, Germany).

    QC/EQA/Proficiency:

    Article Title: Oral microbiome and postmenopausal breast cancer risk: findings from the NIH-AARP diet and health study
    Article Snippet: .. A total of 15 DNA extraction batches were created with matched case-control pairs included in the same batch along with 6 quality control samples: two bacterial artificial communities (the Microbiome Quality Control Project [MBQC] oral artificial community [ ] and the ZymoBiomics Microbial Community Standard [Zymo Research, Irvine, CA]), a mouthwash from a generous donor, two extraction replicates and one extraction blank (TE buffer). .. DNA extractions were performed at the Cancer Genomics Research Laboratory (CGR) at the National Cancer Institute (NCI) in Rockville, Maryland, using the DNeasy PowerSoil Pro kit on the QIAcube HT automated purification system (Qiagen, Hilden, Germany).

    Microbiome:

    Article Title: Oral microbiome and postmenopausal breast cancer risk: findings from the NIH-AARP diet and health study
    Article Snippet: .. A total of 15 DNA extraction batches were created with matched case-control pairs included in the same batch along with 6 quality control samples: two bacterial artificial communities (the Microbiome Quality Control Project [MBQC] oral artificial community [ ] and the ZymoBiomics Microbial Community Standard [Zymo Research, Irvine, CA]), a mouthwash from a generous donor, two extraction replicates and one extraction blank (TE buffer). .. DNA extractions were performed at the Cancer Genomics Research Laboratory (CGR) at the National Cancer Institute (NCI) in Rockville, Maryland, using the DNeasy PowerSoil Pro kit on the QIAcube HT automated purification system (Qiagen, Hilden, Germany).

    Extraction:

    Article Title: Oral microbiome and postmenopausal breast cancer risk: findings from the NIH-AARP diet and health study
    Article Snippet: .. A total of 15 DNA extraction batches were created with matched case-control pairs included in the same batch along with 6 quality control samples: two bacterial artificial communities (the Microbiome Quality Control Project [MBQC] oral artificial community [ ] and the ZymoBiomics Microbial Community Standard [Zymo Research, Irvine, CA]), a mouthwash from a generous donor, two extraction replicates and one extraction blank (TE buffer). .. DNA extractions were performed at the Cancer Genomics Research Laboratory (CGR) at the National Cancer Institute (NCI) in Rockville, Maryland, using the DNeasy PowerSoil Pro kit on the QIAcube HT automated purification system (Qiagen, Hilden, Germany).

    Article Title: Same-day hybrid capture workflow for diagnosis and surveillance of respiratory pathogens
    Article Snippet: To this end, the ZymoBIOMICS DNA/RNA Miniprep Kit for nucleic acid extraction was replaced with the Quick-DNA/RNA Pathogen Miniprep Kit (Zymo Research, R1042), and samples were processed according to the manufacturer’s instructions. .. To evaluate the performance of both extraction kits, we used the ZymoBIOMICS Microbial Community Standard (Zymo Research, D6300). .. Library preparation of the extracts was performed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, E7805) and sequenced using MiSeq Reagent Kit v2 (300 cycles; Illumina, MS-102-2002) For cDNA synthesis, the RPIP cDNA synthesis workflow was replaced with a rapid protocol based on LunaScript RT SuperMix (New England Biolabs, E3010) for first-strand synthesis and Sequenase Version 2.0 DNA Polymerase (Thermo Fisher Scientific, 70775Y) for second-strand synthesis.

    Polymerase Chain Reaction:

    Article Title: Phylloplane fungal community composition shifts with Austropuccinia psidii infection status and reveals candidate fungal antagonists for future biocontrol research.
    Article Snippet: The PCR amplification was performed in a T100 thermal cycler (Bio-Rad) using the following conditions: an initial denaturation at 94°C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72°C for 60 s, with a final extension at 72 °C for 5 min and a hold at 4 °C. .. All PCR runs included a no-template negative control, and the ZymoBIOMICS microbial community standard (Zymo Research, Irvine, CA, USA) was used as a positive control. .. PCR products were visualised by electrophoresis on 1.2% agarose gels run at 100 V for 35 min and stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific, Australia).

    Negative Control:

    Article Title: Phylloplane fungal community composition shifts with Austropuccinia psidii infection status and reveals candidate fungal antagonists for future biocontrol research.
    Article Snippet: The PCR amplification was performed in a T100 thermal cycler (Bio-Rad) using the following conditions: an initial denaturation at 94°C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72°C for 60 s, with a final extension at 72 °C for 5 min and a hold at 4 °C. .. All PCR runs included a no-template negative control, and the ZymoBIOMICS microbial community standard (Zymo Research, Irvine, CA, USA) was used as a positive control. .. PCR products were visualised by electrophoresis on 1.2% agarose gels run at 100 V for 35 min and stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific, Australia).

    Positive Control:

    Article Title: Phylloplane fungal community composition shifts with Austropuccinia psidii infection status and reveals candidate fungal antagonists for future biocontrol research.
    Article Snippet: The PCR amplification was performed in a T100 thermal cycler (Bio-Rad) using the following conditions: an initial denaturation at 94°C for 3 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72°C for 60 s, with a final extension at 72 °C for 5 min and a hold at 4 °C. .. All PCR runs included a no-template negative control, and the ZymoBIOMICS microbial community standard (Zymo Research, Irvine, CA, USA) was used as a positive control. .. PCR products were visualised by electrophoresis on 1.2% agarose gels run at 100 V for 35 min and stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific, Australia).



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